The 5′ untranslated region of fruA m

The 5′ untranslated region of fruA mRNA in Myxococcus xanthus positively regulates the expression of its own gene

Myxococcus xanthus provides an excellent model organism for studying the mechanism of multicellular morphogenesis. The mRNA for FruA, a transcription factor essential for the development of M. xanthus, contains a very long 5′-UTR consisting of 235 nucleotides. Using lacZ as a reporter gene, two fruA-lacZ translational fusions retaining or lacking the fruA 5′-UTR were constructed and separately integrated at phage Mx8 attachment site (attB) in M. xanthus chromosome. Deletion in 5′-UTR between nucleotides from +4 to +220 abolished fruA-lacZ expression during development, indicating that the 5′-UTR is essential for the induction of fruA. Prediction of the RNA secondary structure of 5′-UTR shows that this region could form an extremely stable three-helix junction structure, which might be a binding site for a regulatory protein or contain a cis-acting element(s) to control fruA expression. Thus, the 5′-UTR of fruA mRNA positively regulates the expression of its own gene.

作 者： WU Qian MAO Xiaohua   作者单位： Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Department of Genetics and Developmental Biology, Southeast University School of Basic Medical Sciences, Nanjing 210009, China  刊 名： 科学通报（英文版）  SCI 英文刊名： CHINESE SCIENCE BULLETIN  年，卷(期)： 2004 49(21)  分类号： Q3  关键词： Myxococcus xanthus   fruA   5′-UTR   development   regulation  

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