木质素高效降解菌血红密孔菌产酶条件研究

木质素高效降解菌血红密孔菌产酶条件研究

Abstract： [Objective]The purpose was to study the optimum conditions of Pycnoporus sanguineus for producing ligninase.[Method]A strain of lignindegrading white-rot fungus was selected from 5 strains of collected fungi and its liguinase production and the optimum conditions for producing ligninolytic enzyme were measured.[Result]It could produce two kinds of ligninase with good thermal stability.Different temperatures,carbon sources,nitrogen sources,acidities,as well as the additions of surfactant had distinct influence on the development of ligniu-degrading enzymes of the fungus.The optimum condition was drawn out:38 ℃,pH=4.5,10.0 g/L glucese,1.0 g/L tartaric acid ammonium.[Conclusion]The aim of research was to provide a basis for liguin degradation in practical production. 作 者： 王丹梅    任大明    李秀娜    石皎    WANG Dan-mei    REN Da-ming    LI Xiu-na    SHI Jiao   作者单位： 沈阳农业大学生物科学技术学院,辽宁沈阳,110161  期 刊： 农业科学与技术(英文版)  ISTIC   Journal： AGRICULTURAL SCIENCE & TECHNOLOGY  年，卷(期)： 2008, 9(1)  分类号： X7  Keywords： White-rot fungus    Lignin degradation    Fermentation condition    Orthogonal test   

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