Homogeneous DNA Detection Based on F

Homogeneous DNA Detection Based on Fluorescence Quenching by Nanoparticles in Single-step Format :Target-Induced Configu

A new strategy for homogeneous detection of DNA hybridization in single-step format was developed based on fluorescence quenching by gold nanoparticles.The gold nanoparticle is functionalized with 5'-thiolated 48-base oligonucleotide(probe sequence),whose 3'-terminus is labeled with fluorescein(FAM),a negatively charged fluo-rescencc dye.The oligonucleotide adopts all extended configuration due to the electrostatic repulsion between nega-tively charged gold nanoparticle and the FAM-attached probe sequence.After addition of the complementary target sequence,specific DNA hybridization induces a conformation change of the probe from an extended stmcture to an arch.1ike configuration,which brings the fluorophore and the gold nanoparticle in close proximity.The fluorescence is efficiently quenched by gold nanoparticles.The fluorescence quenching efficiency is related to the target concen.tration,which allows the quantitative detection for target sequence in a sample.A linear detection range from 1.6 to 209.4 nmoI/L Was obtained under the optimized experimental conditions with a detection limit of 0.1 nmol,L.In the assay system,the gold nanoparticles act as both nanoscaffolds and nanoquenchers.Furthermore,the proposed strategy.in which only two DNA sequences arc involved,is not only different from the traditional molecular bea-cons or reverse molecular beacons but also different from the commonly used sandwich hybridization methods.In addition,the DNA hybridization detection was achieved in homogenous solution in a single-step format,which al-lows real.time detection and quantification with other advantages such as easy operation and elimination of washing steps.

作 者： ZHANG,Songbai WU,Zaisheng XIE,Ming SHEN,Guoli YU,Ruqin   作者单位： State Key Laboratory for Chemol Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University.Changsha,Hunan 410082,China  刊 名： 中国化学（英文版）  SCI 英文刊名： CHINESE JOURNAL OF CHEMISTRY  年，卷(期)： 2009 27(3)  分类号： O6  关键词： DNA hybridization   fluorescence quenching   gold nanoparticle   fluorescence resonance energy transfer   configuration transform  

【Homogeneous DNA Detection Based on F】相关文章：

Adaptive Impedance-controlled Manipulator Based on Collision Detection04-26

Detection of Doping with rhGH04-26

Adapting Non-Task-Based Materials to Fit a Task-Based Framework04-27

DNA电脑04-26

Physically based simulation of Buddha Glory04-26

DNA聚合酶与DNA复制的忠实性04-26

Temperature and precipitation in Mongolia based on dendroclimatic investigations04-27

枸杞线粒体DNA的提取04-26

dna粗提取与鉴定06-27

Occupant evacuation model based on cellular automata in fire04-27