Ethanol disrupts the formation of hy

Ethanol disrupts the formation of hypochord and dorsal aorta during the development of embryonic zebrafish

Exposure to ethanol during human embryonic period has severe teratogenic effects on the cardiovascular system. In our study, we demonstrated that ethanol of gradient concentrations can interfere with the establishment of circulatory system in embryonic zebrafish. The effective concentration to cause 50% malformations (EC50) was 182.5 mmol/L. The ethanol pulse exposure experiment displayed that dome stage during embryogenesis is the sensitive time window to ethanol. It is found that 400 mmol/L ethanol pulse exposure can induce circulatory defects in 43% treated embryos. We ruled out the possibility that ethanol can interfere with the process of hematopoiesis in zebrafish. By employing in situ hybridization with endothelial biomarker (Flk-1), we revealed that ethanol disrupts the establishment of trunk axial vasculature, but has no effect on cranial vessels. Combined with the results of semi-thin histological sections, the in situ hybridization experiments with arterial and venous biomarkers (ephrinB2, ephB4) suggested that ethanol mainly interrupts the development of dorsal aorta while has little effect on axial vein. Further study indicated the negative influence of ethanol on the development of hypochord in zebrafish. The consequent lack of vasculogenic factors including Radar and Ang-1 partly explains the defects in formation and integrity of dorsal aorta. These results provide important clues to the study of adverse effects of ethanol on the cardiovascular development in human fetus.

作 者： QIAN Linxi WANG Yuexiang JIANG Qiu ZHONG Tao SONG Houyan   作者单位： Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School of Fudan University, Shanghai 200032, China  刊 名： 中国科学C辑（英文版）  SCI 英文刊名： SCIENCE IN CHINA (LIFE SCIENCES)  年，卷(期)： 2005 48(6)  分类号： Q95  关键词： Ethanol   zebrafish   dorsal aorta   hypochord   vasculogenesis  

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