First isolation and characterization

First isolation and characterization of a new species of Vibrio from diseased flounder (Paralichthys olivaceus Temminck

Appropriate pathogenic bacteria were isolated from diseased (or dead) flounder (Paralichthys olivaceus L) occurring in a mariculture farms of Qinhuangdao, which all diseased fishes expressed bacterial septieaemia. The phenotypic information of the 12 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemical characteristics, and the mole fraction G+C ratio of the DNA for representative strain. The results show that the isolates belong to a new species of Vibrio, and are designated as Vibrio qinhuangdaora sp.nov. by the area from which the diseased flounders are collected (Qinhuangdao) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. At the same time,studies on the serum homology of isolates, and pathogenicity of isolates were conducted by experimental infection. The results show that all the isolates are of serologic similarity, and the isolates have strong pathogenicity to flounder. The studies can extensively reflect the main biological properties of this new species of Vibrio.

作 者： ZHANG Xiaojun FANG Hai Chen Cuizhen Zhan Wenbin Jin Xiaomin Wang Xiuyun   作者单位： ZHANG Xiaojun(Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600,China;Laboratory of Pathology and Immunology of Aquatic Animals, Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China)

FANG Hai,Chen Cuizhen,Jin Xiaomin,Wang Xiuyun(Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600,China)

Zhan Wenbin(Laboratory of Pathology and Immunology of Aquatic Animals, Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China) 

刊 名： 海洋学报（英文版）  SCI 英文刊名： ACTA OCEANOLOGICA SINICA  年，卷(期)： 2005 24(3)  分类号： P7  关键词： flounder (Paralichthys olivaceus L)   Vibrio qinhuangdaora sp. nov.   biological properties  

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