Genetic Analysis and Mapping of TWH

Genetic Analysis and Mapping of TWH Gene in Rice Twisted Hull Mutant

A mutant with twisted hulls was found in a breeding population of rice (Oryza sativa L.). The mutant shows less grain weight and inferior grain quality in addition to twisted hulls. Genetic analysis indicated that the phenotype of mutant was controlled by a single recessive gene (temporarily designated as TWH). To map the TWH gene, an F2 population was generated by crossing the twh mutant to R725, an indica rice variety with normal hulls. For bulked segregant analysis, the bulk of mutant plants was prepared by mixing equal amount of plant tissue from 10 twisted-hull plants and the bulk of normal plants was obtained by pooling equal amount tissue of 10 normal-hull plants. Two hundred and seven pairs of simple sequence repeat (SSR) primers, which are distributed on 12 rice chromosomes, were used for polymorphism analysis of the parents and the two bulks. The TWH locus was initially mapped close to the SSR marker RM526 on chromosome 2. Therefore, further mapping was performed using 50 pairs of SSR primers around the marker RM526. The TWH was delimited between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provide the foundation for further fine mapping, cloning and functional analysis of the TWH gene.

作 者： LI Jin-bo XIA Ming-yuan WAN Bing-liang DU Xue-shu ZHA Zhong-ping YU Da-zhao QI Hua-xiong   作者单位： LI Jin-bo,XIA Ming-yuan,WAN Bing-liang,DU Xue-shu,ZHA Zhong-ping,QI Hua-xiong(Food Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China)

YU Da-zhao(Plant Protection and Soil and Fertilizer Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China) 

刊 名： 水稻科学（英文版）  英文刊名： RICE SCIENCE  年，卷(期)： 2009 16(1)  分类号： S5  关键词： rice   twisted-hull mutant   genetic analysis   gene mapping